Background: Through a response of the maternal neuro-endocrine-immune system, stress has long been thought to be a potential cause of unexplained recurrent miscarriage. Objective: Employing both psychometric and biochemical measurements, to determine the effect of stress in recurrent miscarriage. Study Design and Setting: A prospective case-control research was carried out between December 2022 and April 2023 at the Babylon Maternity and Pediatric Teaching Hospital. Patients and Methods: One hundred women, aged twenty to forty, were separated into the following two groups: Fifty women in Group A with unexplained recurrent miscarriage (defined as three or more spontaneous miscarriages occurring back-to-back at 24 weeks or less) were included in the study. Group B consists of fifty healthy female volunteers who had never experienced a miscarriage, infertility, pituitary condition, psychiatric disorder, or sadness. The participants were measured for their height, weight, body mass index, LH, FSH, prolactin and low dose dexamethasone suppression test, among other measurements. Three pre-designed questionnaire packages, including the Perceived Stress Scale, the Positive and Negative Affect Schedule and the Fertility Problem Inventory, were given to qualified candidates to complete. Result: Compared to control women, women who had recurrent miscarriages experienced higher levels of psychological stress. Serum cortisol and pregnancy outcomes did not correlate (p>0.05). Cortisol levels and any one particular psychometric measure did not significantly correlate with one another (p>0.05Low plasma prolactin levels in the physiologically normal range have been linked to an increased risk of subsequent miscarriages in women who have already experienced one. The results of the psychometric tests did not substantially correlate with plasma prolactin, serum cortisol, or any of the other variables (p>0.05). Conclusion: Stress levels were higher in recurrent miscarriage women than in control women. There is no correlation between cortisol levels and success rates of conception in women who have recurrent miscarriage. Women who experience unexplained recurrent loss have a higher chance of miscarriage when their prolactin levels are low although the actual cause of the observation is unknown, it appears likely that maintaining successful pregnancies requires a sufficient level of prolactin concentration.