The aim of this study was to obtain a significant amount of Glycoprotein created from an isolated local source. L. acidophillus isolated from healthy Iraqi women's clinical samples (vagina) and identify by api 50 CH system . The anti-biofilm activity of partial purified Glycoprotein increases in resistance to S. aureus and P. aeruginosa were seen as the glycoprotein concentration changed.The results indicated how effectively Glycoprotein in (MSM) penetrated the biofilm and killed bacteria. The maximum efficiency against S. aureus was 82%, and against P. aeruginosa it was 61% Inhibition zones of antibiotics at various concentrations have been measured some resist and other sensitive against S. aureus and P. aeruginosa, according to research on the combined action of Glycoprotein with some antibiotics against pathogenic clinical isolates. After the addition of Glycoprotein with antibiotics, the range of inhibition zones was increased and the isolate become sensitive.