Anthelmintic Activity of Dianthus Caryophyll Clove Extracts against Haemonchus Contortus, In Vitro
The study was composed using aqueous and alcoholic extracts of Dianthus caryophyll. When applied at full strength (100%), the aqueous extract of carnation flowers was found to have a substantial impact on nematodes, eliminating them within approximately (1.52 ± 0.1) hrs., while the concentration 25% showed the least effect compared to the negative control group. Both concentrations 75 and 100% of the alcoholic extract of the same plant showed a significant effect in a period of (2.30 ± 0.09) and (1.58 ± 0.23) hrs., respectively, compared with the other concentrations or the negative control group. This study is considered one of the first studies in Iraq that used a scanning electron microscope (SEM) to investigate the morphological and structural transformations in H. contortus treated by using extracts of the plant under study. It showed deformation in the furrows and annuli of the cuticle, and longitudinal wrinkles on the outer surface of the treated worms, rupture areas also found on the outer surface of the worms compared with the control group. It can be concluded that the extracts of D. caryophyll has the potential to be used as an alternative anthelminticbe instead of using chemical treatments that may have side effects on the animals or that the worms have become resistant to.