Background: The objectives of the study were to establish the prevalence of musculoskeletal disorders (MSDs) among the nurses in Kenya, identify risk factors and characterize the MSDs according to anatomical sites. Materials and Methods: A cross-sectional study design was performed to analyze MSDs among nurses at Kenyatta National Hospital (KNH). A Self-administered questionnaire was used to gather information from 314 randomly selected nurses from the six departments that handle patients. Two hundred and forty-four questionnaires were completed and returned, representing a 78% response rate and the highest response rate was found in the medical department. Results: The study established that the prevalence of reported incidences of MSDs among the nurses was 74.2%. The study further established that physical factors involving poor posture, lifting of heavy patients and use of excessive force were the most apparent ergonomic aspects precipitating MSDs (50%). The other ergonomic aspects identified were the structural lay out of work place (37%) and work organization (13%). The most vulnerable parts of the body were found to be the back, feet and shoulders, revealing a rate of 32.5, 21.5 and 20.4%, respectively. The most vulnerable age among nurses was found to be in the age bracket of 35 to 44 years. Conclusion: This research established that prevalence of MSDs is high, where 74.2% of all the nurses sampled are affected. This is a high number and required both administrative and technological interventions.