Background: The present study was done to evaluate the pattern of various clinical specimens from the patients admitted in intensive care units of a tertiary care hospital, their positivity and isolated organisms. Material and Methods: The present study was conducted in the department of Microbiology Indira Gandhi Medical College and Hospital where in the various clinical samples received from various ICUs were analysed. The duration of the study was from 14^th January 2019 to 13^th January 2020. Results: A total of 2458 samples were processed out of which 766 (31.16%) were found to be culture positive, sterile were 1463 (59.52%) and contaminants were found in 229 (9.32%). Among all isolates gram negative bacteria were most common, followed by Candida spp. and gram positive bacteria. Maximum number of samples obtainedwere of blood (52.31%).Most common culture positive clinical specimen in the present study was pus (63.49%) followed by endotracheal secretions (59.31%). Gram negative bacteria (91.96%) were predominant in endotracheal secretions, gram positive bacteria (100%) predominated in CSF, and Candida (53.58%) was predominant in blood samples. The most common bacteria isolated in the present study were Klebsiella pneumoniae followed by Non fermenter group of organism. Klebsiella pneumoniae was also found to be the most common isolate from blood, endotracheal secretions, pus and drain whereas in urine Enterococcus faecium and Escherichia coli were found to be predominant isolates. Conclusion: Maximum number ofsamples obtained were of blood and the Most common culture positive clinical specimen in the present study was pus followed by endotracheal secretions.

Background: The profile of organisms causing ICU infections varies widely one country to the other, one hospital to another and even amongst different ICUs of the same hospital.The present study attempts to know the bacteriological profile of bacterial isolates from ICU patients in our institution. Material and Methods: The present study was conducted in the department of Microbiology Indira Gandhi Medical College and Hospital where in the clinical samples received from various ICUs were analysed for bacteriological profile. The duration of the study was from 14^th January 2019 to 13^th January 2020. Results: The age distribution of the patients whose samples were sent was age ranged from 1day to 90 years. Numbers of males were more compared to females. A total of 2458 samples were processed out of which 766 (31.16%) were found to be culture positive, sterile were 1463 (59.52%) and contaminants were found in 229 (9.32%). Among all isolates gram negative bacteria were most common, followed by Candida spp. and gram positive bacteria. Positivity rate was maximum from general ICU 58.36% followed by sick neonatal care unit 25.06% and lowest positivity rate was seen from respiratory ICU 16.66%.The percentage of gram negative bacteria were maximum from HDU (80%), gram positive bacteria were found to be maximum from CTVS-ICU (27.27%) and Candida were maximum from pediatric ICUs (83.08%). In pediatric ICUs highest percentage of Candida spp. were isolated from NICU (64.28%) followed by PICU (60.14%) and SNCU (60%).From GICU Klebsiella pneumoniae was the most common isolate followed by Non fermenter group of organism. Klebsiella pneumoniae was also the commonest isolate from HDU, SNCU and NICU. Whereas Non fermenter group of organism was common in RICU and CTVS ICU. In PICU Escherichia coli was the most common isolate.Conclusion: A periodic surveillance of the ICUs is thus essential as many of the pathogens are not incubating in the patient at the time of admission but are transmitted by the hands of the treating physicians and other inanimate objects that are used during invasive procedures.