The goal of this study was to determine the antioxidant, antimicrobial and anti-inflammatory capacity of oven-dried methanolic extract (OME) of Moringa oleifera (M. oleifera) leaves. OME showed promising total phenolic content (2.6783 ± 0.0555 mg/g, 0.2678 ± 0.0055 mg/g, 0.0586 ± 0.0037 mg/g) and total flavonoid content (0.8079 ± 0.0050 mg/g, 0.0808 ± 0.0005 mg/g, 0.0140 ± 0.0018 mg/g) with tested concentration of 100 mg/mL, 10 mg/mL and 2 mg/mL. Ferric reducing activity (5.6515 ± 0.0983 mM Fe²⁺, 2.0751 ± 0.0410 mM Fe²⁺, 0.6015 ± 0.0128 mM Fe²⁺) and DPPH radical scavenging activity (87.99 ± 0.24%, 16.21 ± 0.17%, 5.82 ± 0.80%) of OME was in correlation with phytochemical compounds. OME also showed antimicrobial activity on E. coli and S. aureus which is comparable to commercial antibiotic (Streptomycin). The IC₅₀ of OME in proteinase inhibition was 2.57 ± 0.02 mg/mL. Overall, OME could be a potential remedy in the management of oxidative stress and microbial infections.