The challenges that happened during the COVID-19 pandemic are preventing infection and synthesizing vaccines against these viral infections. Thyroid peroxidase is antithyroid microsomal antibodies. The presence of antibodies to thyroid peroxidase pointing to an autoimmune disorder. Anti-sperm antibodies  are identified in sera of women sub-fertility for unknown reasons.  Materials and methods : Biochemical measurements of thyroids hormones levels by AFIAS-10 instrument. Anti-TPO by Maglumi 800 instrument and Anti-sperm antibodies measured by ELISA kit from My Biosource.  Results & Discussion : There were no significant changes in T3 and T4 levels . A highly increase in TSH and anti-TPO levels in women previous infected and vaccinated group against COVID-19 group compared with women don’t infected and without vaccination group and infected women without vaccination group, and a significant increase  in infected women without vaccination group compared with women don’t infected and without vaccination group. There was a highly significant increase in anti- sperm ng/mL concentration in women previously infected and vaccinated against COVID-19 group compared with women not infected and without vaccination and infected women without vaccination group.