srjmsSRJMSSRJMS2788-9483https://doi.org/10.47310/srjms.2022.v02i02.007Anti-Biofilm Activity of Trigona Honey against Gram-Positive BacteriaGoljameenMidhatAbdullaOzdanAkramGhareebHusseinSaberQaderIn recent years, the continued unplanned application of antibiotics has led to the up growthof resistance to some bacteria, increasing the global burden of infectious disease. Therefore there is an urgent need for actual research on antimicrobial alternatives. This study was conducted to appreciate the effectiveness of Trigona honey (TH) in preventing biofilm formation as well as inhibiting bacterial growth of some common gram-positive bacteria. The biofilm-inhibiting effect of Trigona honey was examined for the following three common bacteria: Staphylococcus aureus, Streptococcus pyogenes and Enterococcus fecalis at different concentrations of 10%, 20% and 40%. The bactericidal/bacteriostatic activity of TH for these three bacteria was adopted by MBC and MIC in vitro assays. The results proved that TH has an inhibitory action upon the biofilm formation of S. pyogenes and E.fecalis at a concentration of 20% (v/v) while that of S. aureus at a concentration of 40% (v/v).It was concluded that 40% Trigona honey has the best cationic bactericidal activity and prevents biofilm formation.