srjmsSRJMSSRJMS2788-9483https://doi.org/10.47310/srjms.2022.v02i01.001Hepatoprotective Effects of Fenugreek Seeds and Curcumin against Hepatotoxicity Induced by Nicotine in Male Albino RatsMohamedOmarAlbashaAzabElsayedManalAbuelkasemElnaifThe present study aimed to investigate the harmful effects of nicotine on serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities and histological structure  of the liver of male albino rats. And evaluate the protective effects of fenugreek seeds and curcumin on hepatotoxicity induced by nicotine in male albino rats. 30 male F-344/NHsd Fischer rats, weighing from 180 to 200g were used in the present study. The animals were divided into five groups (6 rats for each); Group I (control group), Group II (nicotine treated group), Group III (nicotine/fenugreek seeds co-administered), Group IV (nicotine/curcumin co-administered) and Group V (nicotine/curcuminand fenugreek seeds co-administered). At the end of the experimentation and 24 hours after the last dose, All animals were anaesthetized with ether and blood samples were collected by heart puncture. The blood samples were collected in clean dry tubes. and allowed to clot at room temperature for about 30 minutes and centrifuged at 3000 rpm for 15 minutes then, serum was separated and kept in a deep freezer at -20◦C until biochemical measurements were carried out. Animals were immediately dissected and small pieces of the liver were quickly removed and fixed in 10% formalin for histological examination. The results showed that the animals treated with nicotine for 4 weeks induced a significant increase in serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities compared with the control group. Also, administration of nicotine to rats induced a severe structural change in the hepatic tissues. Co-administration of nicotine with fenugreek and/or curcumin caused improvement in serum liver function and histological structure of the liver when compared with nicotine group. It can be concluded that nicotine had a strong effect on the liver function and histological structure of the liver. The ingestion of fenugreek and/or curcumin prevent the hepatoxicity induced by nicotine.  The current study suggests that fenugreek and curcumin may be useful in combating free radical-induced hepatotoxicity induced by nicotine.