iarjmsIARJMSIARJMS2708-3594https://doi.org/10.47310/iarjms.2021.v02i02.040Find Out the Determination in Intestinal and Urinary Tract Infection by Detection Molecular for Gene HlyaAbbasAbdul Wahhab JumaahAl-SalihiTwo hundred eighteen samples were collected from Yarmouk Hospital, Iraq, Baghdad. In this study, a gene hlyA is partially investigated and detected by relying on the PCR assay, and the design was also based on the NCBI-Gen Bank published sequence, Escherichia coli strain BAU-MH4 HlyA (hlyA) gene. The study specialized in urological patients and identified the hlyA gene by relying on molecular detection and statistical analysis to analyze data and demographic information; the mean Sd was found for age 11.78±2.9. Molecular detection of the hlyA gene shows that 11 isolates were produced from E. coli and divided into eight isolated from urine and three from diarrea. Finally, we conclude on the presence of the gene in E. coli bacteria, in a very high percentage.