iarjcmbIARJCMBIARJCMB2789-6005https://doi.org/10.47310/iarjcmb.2023.v03i02.002Experimental Studies, Characterization and Biological Activity of Azo-azomethin Sulfonamide Derivatives And its Iron (III) ComplexMohammed.Abdulhussin.EnadA trivalent Iron (III) metal chelates of sulfonamide-derived Azo-azomethine (HTDB), as ligand. The Azo-azomethine compound HTDB was synthesized using a condensation reaction of 4-((2-hydroxy-4-oxopent-2-en-3-yl)diazenyl) benzenesulfonamide and p-toluidine, resulting in a magnificent yield. The ligand connected with this transition metal through the oxygen of the hydroxyl group and the nitrogen of azomethine, acting as a bidentate. By using a variety of physical, spectral and analytical techniques. Physical (melting point, color, yields, solubility, magnetic susceptibility and conductance measurements), spectral (UV–visible, Fourier transform infrared spectroscopy, 1H NMR, 13C NMR and mass analysis) and analytical (CHN analysis) methods were used to determine the structures of the ligand and their transition metal complex. They expertly established the structures of the ligand HTDB and their transition metal complex.. Finally, the synthesized compounds were tested for antibacterial activity against strains of bacteria (Escheria coli and Streptococcus aureus) and fungi (Candida albicans).. The significant outcomes of these experiments demonstrated the bioactivity of the ligands and the transition metal complexes they had.