iarjalsIARJALSIARJALS2708-5104https://doi.org/10.47310/iarjals.2024.V05i01.083Study of the integration between mineral, organic and biological fertilization on the growth of Trigonella foenum-graecum and accumulation of TrigonellineAhmedShakerAL-DuhamiNasserFahemYasirDepartment of Crop Sciences, College of Agriculture, Wasit University, IraqThe experiment was applied in the College of Agriculture, Wasit University. Its aim was to study the integration between mineral, organic and biological fertilization on the growth of Trigonella foenum-graecum and the accumulation of Trigonelline. A randomized complete block design (RCBD) has been used in this experiment with three replications. The means were compared according to the least significant differences (LSD) test at a 5% level. The experiment included 7 treatments, which consisted of adding organic, biological, and chemical fertilizer either alone or combined with half of the recommended chemical fertilizer. Organic and biological fertilizers were added once preparing the soil. For chemical fertilizers, the first addition was 20 days after transplanting, and the second was a month after the first addition. The results were compared with the chemical fertilization treatment and with the control treatment. The results of the experiment have showed that the T7 treatment (organic fertilizer + biofertilizer 50% chemical fertilizer) was significantly differences in most of the traits of vegetative including growth, yield, protein increase, and Trigonelline, except for the traits of number of branches and number of pods. It was the highest value in the chemical fertilization treatment, with non-significant differences with the T7 treatment. It is important to find a fertilization program that is friendly to the environment and human health and provides results that are close to or superior to the results of chemical fertilizer.