iarjpIARJPIARJP2709-9512https://doi.org/10.47310/iarjp.2022.v03i01.003Optimization of Biosurfactant Production from Pseudomonas Aeruginosa VB MCC 4751 Isolated from Sea Water Near Uran BeachVidulaBinduVenkatHamdePranjaliYedewarDepartment of Microbiology, Fergusson College, IndiaDepartment of Microbiolgy, Yogeshwari Mahavidyalaya, Ambajogai, IndiaDepartment of Physics, Savitribai Phule Pune University, IndiaOptimization of media components and maintaining suitable growth conditions are the most important steps for the improved production of biosurfactants. In the present study, Pseudomonas aeruginosa VB MCC 4751 isolated from sea water near Uran beach, near ONGC plant, Navi Mumbai, was studied for improved biosurfactant production. Effects of different carbon and nitrogen sources, sodium chloride, pH and temperature on biosurfactant production by Pseudomonas aeruginosa VB MCC 4751 were studied. 1% sodium chloride was found to be suitable for the growth and biosurfactant production was observed within 24 hours of incubation in the shake flask condition at 280C. Using glucose as a carbon source, maximum surface tension reduction up to 44.3 mN/m (69.74 mN/m to 25.39 mN/m) and 60% of emulsification index was observed. Sodium nitrate was found to be the most suitable for biosurfactant production with surface tension reduction of 43.5 mN/m (70.50 mN/m to 27.08 mN/m) and 63.33% of emulsification index. pH 7 and temperature of 30 0 C was found to be optimum (surface tension reduction readings of 27.32 mN/m and 27.8 mN/m respectively) for biosurfactant production though the given isolate was found to produce biosurfactant over a wide range of pH and temperature. Marine bacteria are adapted to adverse environmental conditions and hence suitable for the production of bioactive compounds on commercial scale.