iarjalsIARJALSIARJALS2708-5104https://doi.org/10.47310/iarjals.2022.v03i02.010Use of Grape Seeds Extract powder (GSEP) to Improve Some of Rams Semen Parameters When Preserved at 5 °C and CryopreservationMohammedAbdulameer RashidAl-SarraySince for a long time of Ancient Civilizations, various types of the grape have been used for medicinal purposes. Recently, many reports were referring that grape seed contains oligomeric proanthocyanidin (OPC). Also, many compounds work as antioxidant that is believed to improve certain cell health. The studies treatments were designed to investigate of using Grape seeds extract powder (G.S.E.) to tris extender of ram semen and their effect on some Iraqi Awassi ram semen trails after preservation for different intervals including cooling storage at 5Cº for 0, 24, 48, 72 Hrs., and post cryopreservation at liquid nitrogen (-196 Cº) for one week. The treatments were designed as follow: G0 (as a control group), G1(0.05%), G2(0.10%, G3(0.15%), and G4(0.2%). The study was conducted at ruminant's research satiation (Baghdad-Iraq). Three local's Iraqi Awassi rams ages 2.5-3 years were selected and trained to collect semen by artificial vagina. Samples collected as two ejaculates/ ram/ week during 4 weeks. The study was carried out from the January to April 2021. The treatments G2 (0.1%) and G3 (0.15%) showed a significant improvement (P<0.01) in motility percentage. On the other side, same treatments were showed a significant difference (P≤0.01) decrease of dead, Abnormal, acrosomal abnormalities percentage compared with other treatments, and control groups after 72 hours at 5Cº and post storage at liquid nitrogen tank for one week. In conclusion, adding of 0.10 or 0.15% of grape seeds extract powder to Tris- extender was recorded some good properties to improve ram semen traits, where semen preserving at 5Cº for 0, 24, 48, and 72 hrs or one-week post cryopreservation in liquid nitrogen.