iajabmsIAJABMSIAJABMS2709-3298https://doi.org/10.47310/iajabms.2023.v04i01.003Stability analysis of gold nanoparticles synthesized using various nutshell extract and it’s in vivo toxicology analysis on adult zebrafishMahalingamJeyakumarDuraiDeepikaAnnamalaiJeyameenakshiSince the wide range of industrial, medicinal, and therapeutic uses, gold nanoparticles (AuNPs) have sparked a lot of interest in the rapid development of nanotechnology. In this study, AuNPs were synthesized using the green approach with Pista (SH-1), Badam (SH-2), Walnut (SH-3), Groundnut (SH-4), and Tamarind (SH-5) nutshell extract in this work. Preliminary confirmation of the AuNPs production was attained by a visual color change from light brown to violet color. The UV Vis spectroscopic study of the AuNPs stability indicates the highest stability in Walnut SH-3 and Tamarind SH-5 up to 37th day at pH 7. UV- Visible Spectroscopy, Energy-dispersive X-ray spectroscopy (EDX) and Transmission electron microscope (TEM) were used to characterize the synthesized AuNPs. Physiochemical characterization of AuNPs exhibited absorbance at 550 nm for synthesized AuNPs nearly for all the extracts used, TEM image revealed that the obtained AuNPs are spherical and EDX analysis showed that obtained AuNPs were in pure form. We performed growth assessment on zebra fishes for up to 5 d by monitoring weight at various concentrations of AuNPs of walnut SH-3. It showed no histological abnormalities at organ levels in adult zebrafish up to 16 days. Our findings provided there is no notable cytotoxic evidence found in the synthesized AuNPs.