iarjalsIARJALSIARJALS2708-5104https://doi.org/10.47310/iarjals.2021.v02i01.020Growth Optimization Condition for the Production of Extracellular Polysaccharide from the Mushroom Agaricus heterocystis-VKJ17 Using Response Surface Methodology and Their Antiangiogenic PropertyJagadishLoganathanSuriyaKShenbhagaramanRamalingamVenkatakrishnanVenkatasubbuKaviyarasanVenkatesanCenter for Advanced Studies in Botany, University of Madras, IndiaDepartment of Microbiology, PRIST Deemed University, IndiaDepartment of Biotechnology, Anna University, IndiaThe quantitative effect of different growth parameters like temperature, pH and dextrose to produce extracellular polysaccharide from the mushroom Agaricus heterocystis (AGH) was studied by using Response Surface Methodology (RSM) three factor Box-Behnken design. The experimental data obtained from this study was exactly fitted with a second-order polynomial equation using multiple regression analysis. The surface contour plots of the model predicted that temperature of 30.9°C, pH 4.9±0.2 and dextrose concentration of 1.99 g/100 ml were the optimum growth condition required for the maximum production of extracellular polysaccharide. The predicted yield of extracellular polysaccharide reached around 2469 µg/ml which was relatively significant with the validation experiment. The extracted polysaccharide was purified partially for NMR studies confirmed the presence of glucan-protein complex. The partially purified polysaccharide under ex vivo condition showed anti-angiogenic property needs further study to screen for antitumor and antimetastatic properties.