iarmsIARMSIARMS2709-3255https://doi.org/10.47310/iarms.2021.v02i02.013Reverse Phase Liquid Chromatographic Method Development and Validation of Peptidase-4 (Dpp-4) Inhibitory DrugRamanujMishraRanjeetSinghBargodeSourabhJainRakehPatelPresent research work to method development and validation of peptidase 4 (DPP-4) as inhibitory drug through RP-HPLC. It was validated for the estimation of Metformin HCL and Sitagliptin in tablet dosage form using HPLC Shimadzu Prominence with UV-Visible SPD 20A Detector and Phenominex C18 (250x4.6 mm, 5µ) column, injection of 20 µl is injected and eluted with the mobile phase of dipotassium hydrogen phosphate buffer and acetonitrile in the ratio 55:45, which was pumped at a flow rate of 1mL at 260 nm. The peak of Metformin HCL and Sitagliptin are found well separated at 4.285 and 7.485 respectively. The developed method was validated for various parameters as per ICH guidelines like Accuracy, Precision, Linearity, Specificity, Ruggedness, Robustness, LOQ and LOD. The analytical method validation of Metformin HCL and Sitagliptin by RP HPLC method was found to be satisfactory and could be used for the routine pharmaceutical analysis of Metformin HCL and Sitagliptin.