iarjalsIARJALSIARJALS2708-5104https://doi.org/10.47310/iarjals.2020.v01i02.003Physiological Characters Associated With Water-Stress Tolerance and Proline Content of Sorghum Cultivars under Water-Stress Conditions in Shambat, SudanDohaALi AL-SmmaniMohamedFaisalElgasimAhmedBadrELdin Abdelgadir MohamadAhmedPhD student. Dept. Crop Science. Faculty of Agriculture, University of Khartoum, SudanProf. of Environmental Physiology; Agronomy Dept. Faculty of Agriculture; University of Khartoum, SudanAssociated Prof. Dept. Crop Science. Faculty of Agriculture, University of Kassala, Sudan.A Greenhouse experiment was conducted at Farm of the Faculty of Agriculture, Sudan University of Science and Technology at Shambat during the period of 17th June to 23rd October 2014. The experimental treatments arranged in completely randomized design with four replications. Six genotypes of sorghum (Sorghum bicolor L. Moench) of Sudanese origin ( Wad ahmed , Bashier, Geshesh and Culom Abu 7 and Kolklo designated as V1, V2, V3 ,V4, V5, and V6 , respectively. Plants were grown in pots and they were subjected to either well-watered (W0) or stressed(W1)conditions. Characters studied were Stomatal conductance, Transpiration rate, Specific flag leaf area SFLA and Relative water content RWC, Water use efficiency WUE, Net assimilation rate NAR and Proline content. The results revealed that water stress and cultivars showed significant differences on (stomatal conductance, transpiration rate, specific leaf area and osmotic adjustments). Moreover the significant decrease in aforementioned traits due to sowing of V2 and V3cultivars under water stress was generally associated with significant decreases in NAR, RWC, WUE and proline content. So, these traits help to identify characters associated with water-stress tolerance of sorghum. In conclusion the two sorghum cultivars V2 andV3 outperformed the other cultivars under the most view of points.