iarjalsIARJALSIARJALS2708-5104https://doi.org/10.47310/iarjals.2020.v01i01.013Isolation screening and optimization of laccase producing fungi from decaying tamarind woodHanslodMubinaSulemanBrijeshShuklaSubstrate flexibility of laccase enzymes makes them highly interesting for various applications such as textile dye bleaching, pulp bleaching, and production of chemicals from lignin, bioremediation & development of biosensors to check the presence of xenobiotics. For industrial applications discovery of novel laccases with different substrate specificities and improved stabilities is important. Microbes that produce laccases have been screened on solid media containing colored indicator compounds such as guaiacol that enable the visual detection of laccase production. Optimized condition for Laccase enzyme production by fungal isolate HM1 was: incubation period- 10 days, pH- 5, carbon source- maltose, nitrogen source- peptone, CuSO4 concentration-50µM and temperature-50°C. This research summarizes that the production of laccase for application in bioremediation was easily achieved by production from cost effective economic process of solid state production from corn cobs, rice bran, wheat bran etc.