srjalsSRJALSSRJALS2788-9386https://doi.org/10.47310/srjals.2024.v04i01.001Effect of different concentration of Eruca sativa on cooled and frozen semen ofArabi ramsAl-AsadiHabbibA.AAlilawyASH.AbbasR.H.Four Arabi insemination rams weighing between 45 and 55 kg were utilized in the study. The rams were grown in one of the local breeders' animal fields in the Basrah Governorate and their ages ranged from 3 to 4 years. The study was conducted between November 2023 and January 2024. Every ram was housed in identical surroundings. Twice a week, between 8.0 and 9.0 a.m., semen was extracted using an artificial vagina and within an hour, it was sent to the lab for analysis. The fresh, undiluted and diluted semen were then examined under a microscope. Using a Computer Assisted Sperm Analysis (CASA) technology, the different sperm motility features were evaluated. The experiment was then designed and the subjects were divided into the three groups listed below: T1 and T2 are the control. In Group C (control), there is only one ingredient: tris-citric egg yolk-glycerol (TCEG). Group T1: Mix 5 milliliters of semen with 0.5 microliters of diluted Eruca sativa extract. To 5 milliliters of semen, add 1 µl of diluted Eruca sativa extract (Group T2). After macroscopic semen analysis in the laboratory, semen samples were diluted 1:1 with Egg Yolk Citrate (EYC) and refrigerated for 24, 48, 72, 96 and 120 hours  at  5°C.  Next,  the  cooled  semen samples were put into 0.5 mL straws and frozen by placing the straws 5 cm above liquid nitrogen (LN2) for a duration of 10 minutes. Following this, the straws were immersed in LN2 and kept in a -196°C LN2 storage tank for additional examination. Semen straws were cryopreserved and then rinsed in 37°C water for 30 seconds to defrost them ten days and one month later. Semen was poured into 5 mL test tubes after the two sealed straw ends were severed with scissors. As a result, according to the results, after cooling, T2 had the highest significant value (p<0.05) and Control had the lowest significant value (p<0.05). Reduced VSL, VAP, LIN, ALH (amplitude of lateral head displacement) and BCF were seen in significantly (p<0.05) sperm frozen samples. It is important to add Eruca sativa to semen extender since the percentage of proliferating motile sperm cells followed a similar trend. The results of post-thawing WOB (wobble) and anomaly percentage indicated that there was no significant difference (p<0.05) in the values of T2 and T1 when compared to other control groups. The findings indicated that T2 and T1 had the lowest values of the VCL curvilinear velocity, VAP average path velocity, WOB wobble, ALH amplitude of Lateral head displacement and abnormality% (non-significant), whereas control had the lowest significant values at post-thawing. According to the results, T2 had the (p<0.05) higher LIN linearity and STR straightness than the other control group.